Overview of CJC-1295 DAC in Laboratory Research
CJC-1295 DAC is a synthetic 30-amino acid peptide analog of growth hormone-releasing hormone (GHRH). Developed to overcome the short half-life of native GHRH, the peptide incorporates a Drug Affinity Complex (DAC) modification that enables reversible binding to serum albumin. This structural feature substantially extends its presence in circulation, making it a reliable tool for sustained-release research models. In laboratory settings, CJC-1295 DAC is utilized exclusively in 体外 以及 体内 (animal) studies to investigate growth hormone (GH) secretory patterns, cellular signaling cascades, and metabolic regulation. It is not designed, authorized, or sold for any human or veterinary clinical application. All usage is confined to controlled experimental environments governed by institutional review.
Chemical Properties and Structural Characteristics
The peptide has an empirical formula of C152H252N42O42 and a molecular weight of approximately 3355 g/mol. It is supplied as a lyophilized powder, appearing white to off-white, and is highly soluble in sterile water or phosphate-buffered saline (PBS) at physiological pH. For extended stability, the lyophilized form should be stored at -20°C or below in a desiccated environment, protected from direct light. Once reconstituted, the solution should be used promptly; long-term storage of the liquid form even at -20°C is not recommended due to potential degradation. The DAC moiety, a maleimidopropionic acid derivative attached via a spacer, confers the albumin-binding property without interfering with receptor recognition, a detail critical for research consistency.
Mechanism of Action in Research Settings
CJC-1295 DAC functions as a highly selective GHRH receptor agonist. Upon administration in laboratory models, it binds to specific receptors on anterior pituitary somatotroph cells, triggering adenylate cyclase activation and cyclic AMP (cAMP) production. This cascade leads to increased synthesis and pulsatile secretion of growth hormone. The unique DAC component covalently attaches to the free thiol group of cysteine‑34 on circulating albumin, forming a stable peptide‑albumin conjugate. This binding slows renal clearance and enzymatic degradation, prolonging the peptide’s bioactive half-life to several days in rodent models, compared to minutes for unmodified GHRH. Researchers use this property to maintain steady-state GH levels, exploring downstream effects on insulin-like growth factor 1 (IGF-1) production, lipid metabolism, and protein synthesis in tissues such as liver and muscle. The extended action also allows dissection of receptor desensitization, feedback inhibition, and diurnal secretory rhythms without frequent intervention.
Advantages of the DAC Modification for Research
The Drug Affinity Complex technology significantly improves experimental design in several ways:
- Reduced handling frequency: In animal models, a single administration of CJC-1295 DAC sustains elevated GH for multiple days. This minimizes injection-related stress and handling artifacts, which can confound metabolic and behavioral endpoints.
- Consistent systemic exposure: Sustained peptide levels attenuate the peak‑trough fluctuations typical of unmodified hormones, yielding more uniform stimulation of target tissues. This consistency is crucial when quantifying dose‑response relationships in long‑term protocols.
- Feasibility of chronic studies: Researchers can model prolonged GH axis activation over weeks without the logistical burden of daily or twice‑daily dosing, preserving animal welfare and improving data reproducibility.
- Reduced inter‑animal variability: By eliminating multiple dosing steps, pharmacokinetic variability introduced by absorption differences between animals is lowered, strengthening statistical power.
These benefits make the DAC-modified peptide especially valuable for investigations into growth, aging, and endocrine cross‑talk.
Considerations for Bulk Purchase of CJC-1295 DAC
When sourcing CJC-1295 DAC for large‑scale research workflows, buyers typically evaluate batch sizes from 10 mg up to 1 g or more, depending on supplier capabilities. A purity specification of ≥98% as determined by reverse‑phase high‑performance liquid chromatography (HPLC) is the industry benchmark. Each shipment should be accompanied by a Certificate of Analysis (CoA) detailing the exact purity, mass spectrometry confirmation of molecular weight, residual solvents, and endotoxin levels. Bulk purchasing often unlocks tiered pricing, but institutions must also account for shipping conditions (cold chain if required) and customs clearance documentation. Engaging directly with the manufacturer or an authorized distributor can ensure continuity of supply and access to technical support for handling and reconstitution protocols.
Purity and Quality Assurance
Robust quality control is non‑negotiable. Analytical techniques should include HPLC for purity quantitation, liquid chromatography‑mass spectrometry (LC‑MS) for identity verification, and amino acid analysis to confirm composition. Endotoxin testing (e.g., LAL assay) and microbial limit tests verify that the product is free from contaminants that could interfere with cell‑based assays or animal physiology. Batch‑to‑batch consistency is maintained through strict adherence to standard operating procedures during synthesis and purification. Researchers are advised to request a sample CoA before committing to a bulk order, and to independently re‑verify purity upon receipt using their own HPLC or MS capabilities, particularly for publication‑quality data.
Storage and Handling Protocols
To preserve structural integrity, lyophilized CJC-1295 DAC should be stored at -20°C or preferably -80°C, in a manual defrost freezer to avoid freeze‑thaw cycles from automatic defrost. Keep the vial in a sealed container with desiccant, protected from light. Upon reconstitution, sterile water for injection or sterile PBS is recommended; the peptide is typically dissolved to a stock concentration of 1‑2 mg/mL. Aliquot the stock solution into single‑use portions to avoid repeated warming and cooling. Never store reconstituted peptide for more than 24 hours at 4°C; for longer storage, snap‑freeze aliquots in liquid nitrogen and store at -80°C, though some loss of activity may occur. All handling must be performed in a laminar flow hood using sterile, pyrogen‑free consumables to prevent environmental contamination.
Regulatory Compliance for Research Peptides
CJC-1295 DAC is designated exclusively for laboratory research purposes. Product labels and packaging must carry explicit statements such as “For research use only. Not for human or veterinary use.” In many jurisdictions, including the United States (under FDA oversight), Europe (EMA), and Asia, importers and end‑users must document that the material will be employed solely in bench‑top or preclinical animal studies approved by an institutional animal care and use committee. When shipping internationally, the commercial invoice and packing list should clearly state the research‑only nature of the consignment to facilitate customs clearance. Buyers are responsible for verifying that their intended use complies with all local, national, and institutional regulations. Any deviation into clinical or diagnostic application is strictly prohibited and may carry legal penalties.
Selecting a Reliable Supplier for Bulk CJC-1295 DAC
A dependable supplier is characterized by transparency and rigorous manufacturing standards. Key selection criteria include:
- Certified quality systems: Look for adherence to Good Manufacturing Practices (GMP) for peptides, ISO 9001 certification, or equivalent quality management frameworks. These indicate controlled production environments and traceable documentation.
- Comprehensive analytical data: Suppliers should provide detailed CoAs for every batch, specifying HPLC purity, MS spectrum, peptide content, and endotoxin units. Some also offer solubility testing data.
- Supply chain reliability: Assess lead times, shipping methods (dry ice or cold packs), and the ability to handle large or recurring orders without batch diversion. A single‑source manufacturer with internal synthesis capabilities often ensures better lot tracking and fewer supply interruptions.
- Customer and technical support: Responsive communication regarding inquiry, protocol advice, and after‑sale support is essential, especially when scaling up from milligram to gram quantities. Ask about peptide storage recommendations and compatibility with your research setup.
- Pricing and terms: Bulk wholesale pricing should be transparent, with discounts clearly tied to order volume. Confirm whether shipping, insurance, and any customs duties are included in the quotation.
Due diligence, including review of independent customer feedback and, if feasible, an on‑site audit, can further mitigate risks when establishing a long‑term procurement partnership.
结论
CJC-1295 DAC serves as a powerful laboratory agonist for probing GHRH‑GH‑IGF‑1 axis biology in controlled experimental systems. Its albumin‑conjugated DAC alteration yields a long‑acting profile that simplifies chronic study designs and improves data consistency. When buying this peptide in bulk wholesale quantities, research institutions, pharmaceutical companies, and authorized distributors must prioritize verified purity (≥98%), rigorous quality assurance documentation, and strict regulatory compliance. Selecting a supplier with transparent manufacturing processes and reliable logistics ensures that your laboratory receives a consistent, high‑quality product capable of supporting reproducible scientific outcomes. For a customized quotation and to discuss your specific research-grade requirements, contact our team. All quotations are provided with the understanding that the product is intended solely for laboratory and animal model investigations, with no implied suitability for human or veterinary diagnostic or therapeutic purposes.
仅限研究用途： This peptide is supplied strictly for in vitro and approved animal research. It is not intended for any clinical or veterinary application. Any statements regarding its properties are based on peer‑reviewed literature and are not to be construed as claims for human or animal use. Please consult your institution’s ethics committee and all applicable regulations before ordering.
