Overview of 5-Amino-1MQ Raw Material
5-Amino-1MQ (5-Amino-1-methylquinolinium) is a selective small‑molecule inhibitor employed in laboratory research to study nicotinamide N‑methyltransferase (NNMT) function. The raw material is supplied as a high‑purity lyophilized powder, prepared under strictly controlled conditions for experimental applications. It is intended solely for in vitro use and biochemical investigation; it is not manufactured for, nor may it be used in, human or veterinary contexts. Laboratories seeking 5-Amino-1MQ raw material for sale can acquire the compound in research‑grade form, accompanied by full documentation to support reproducible scientific work.
Chemical Structure and Properties
The molecular formula and exact molecular weight of 5‑Amino‑1MQ are documented on the batch‑specific Certificate of Analysis provided with each shipment. The compound is a quaternary ammonium salt, typically supplied as the iodide counter‑ion, which confers favorable solubility characteristics for cell‑based and biochemical assays.
Stability studies indicate that the lyophilized powder remains structurally intact when stored desiccated at –20 °C and protected from light. Under these conditions, long‑term integrity has been confirmed; short‑term excursions at ambient temperature during transit do not compromise the material. Researchers requiring detailed degradation profiles may request the comprehensive stability report.
- Handling: Hygroscopic; open under inert gas and re‑seal promptly after use.
- Solubility: Soluble in standard laboratory solvents such as dimethyl sulfoxide (DMSO), water, and phosphate‑buffered saline (PBS). Solubility limits and preparation recommendations are supplied in the product information sheet.
- Compatibility: Data on solvent compatibility and recommended diluent for cell‑culture applications are included with each order.
Applications in Laboratory Research
5‑Amino‑1MQ serves as a critical tool compound in studies of methylation‑driven metabolic pathways. Because of its high selectivity for NNMT over other methyltransferases, it is widely adopted to dissect nicotinamide metabolism and S‑adenosylmethionine‑dependent signaling.
Common experimental uses include:
- Investigating NNMT’s role in adipocyte energy handling and lipid accumulation in vitro.
- Employing the inhibitor in biochemical binding assays to characterize enzyme–substrate and enzyme–inhibitor kinetics.
- Applying it as a reference compound in receptor‑associated signaling studies where NNMT activity may modulate local methyl‑donor pools.
- Using the molecule as a chemical probe to analyze peptide‑modifying enzyme networks and methylation‑sensitive protein interactions.
All such applications are confined to cell‑free systems or cultured cell models; the compound is never used in whole‑organism studies.
Cell Culture and In Vitro Studies
When introduced into cultured cell lines, 5‑Amino‑1MQ allows researchers to observe downstream metabolic and transcriptional changes triggered by NNMT inhibition. The compound is reconstituted in sterile vehicle and applied under aseptic conditions. Concentration parameters and incubation periods are determined by the lead investigator based on the specific cellular model and endpoint readout, typically following published in vitro protocols. Experimental designs are validated within the individual laboratory, and the compound is never administered to living animals or human subjects.
Adherent and suspension cell lines have been treated with the molecule to measure outcomes such as altered NAD+ levels, changes in histone or DNA methylation status, and shifts in differentiation markers. All work is performed under biosafety level‑appropriate containment, and any results are interpreted strictly within the context of preclinical mechanism exploration.
Quality Assurance and Sourcing
Every production batch of 5‑Amino‑1MQ raw material undergoes rigorous analytical characterization before release. The quality‑control panel includes reversed‑phase high‑performance liquid chromatography (RP‑HPLC) for purity assessment and liquid chromatography–mass spectrometry (LC‑MS) for identity confirmation. Typical purity exceeds 98% as measured by HPLC at 254 nm, with no single impurity above 1%.
Additional testing may cover:
- Elemental analysis – to verify salt form stoichiometry.
- Residual solvent analysis – ensuring compliance with International Council for Harmonisation (ICH) guidelines for research‑grade materials.
- Heavy metal screening – confirming that levels of toxic metals remain below the limits set for laboratory reagents.
A Certificate of Analysis detailing batch‑specific results is included with every order. The compound is synthesized in facilities that adhere to strict quality management systems, with process controls spanning raw material qualification, in‑process monitoring, and final lot release. Manufacturing environments are routinely audited to maintain high standards of purity and consistency.
Ordering and Shipping Information
5‑Amino‑1MQ is offered in standard research quantities of 10 mg, 50 mg, and 100 mg, supplied in amber glass vials sealed under argon. Larger aliquot sizes can be accommodated upon request for institutional customers requiring bulk product for high‑throughput screening.
Shipping is carried out under ambient conditions, with the lyophilized powder having been validated to withstand temperature fluctuations typically encountered during international transit. Each package includes the relevant safety data sheet (SDS), Certificate of Analysis, and a packing list that facilitates customs clearance. Global logistics are arranged to serve authorized research institutions, pharmaceutical R&D centers, and verified distributors. Cold‑chain shipping can be organized if required by the recipient’s protocol, though it is not necessary for product stability in the short term.
Prospective purchasers are screened to confirm that the product will be used exclusively for legitimate laboratory research. No orders are fulfilled where the intended application falls outside the permitted research‑use boundary.
Research use only: This product is designed and supplied for in vitro laboratory research exclusively. It is not for use in humans, animals, or any diagnostic, prophylactic, or other non‑research purpose. All purchasers must agree to these terms before shipment.
For research use only. Not for human or veterinary use.
