Introduction
AOD-9604 is a synthetic peptide fragment derived from the C‑terminus of human growth hormone (hGH), specifically residues 177–191. Its structure retains a short 8‑amino‑acid sequence (Tyr‑Arg‑Ile‑Gln‑Leu‑Ala‑Gly‑Leu‑NH₂) that has drawn significant interest in preclinical investigations of metabolic regulation and cell signaling pathways. Because AOD-9604 represents only a portion of the full-length hGH molecule, laboratories can study its interaction with receptors and downstream effects without interference from the broader hormonal activities of intact hGH.
For dependable experimental outcomes, the purity of the peptide is paramount. Contaminants—whether synthesis by‑products, truncated sequences, or residual solvents—can introduce unwanted variables that obscure data interpretation. Research‑grade AOD-9604 with a purity ≥99% (determined by HPLC) ensures that each experiment begins with a well‑characterized starting material, thereby improving reproducibility and reducing the risk of false‑positive or false‑negative observations.
As a specialized B2B supplier, we provide AOD-9604 exclusively to qualified research institutions, pharmaceutical companies, and authorized distributors. All products are intended strictly for laboratory research applications, including in vitro assays and controlled in vivo studies in model organisms. Our materials are not manufactured, labeled, or approved for any diagnostic, therapeutic, or veterinary purpose. By adhering to this principle, we support the scientific community with reliable reagents while maintaining full regulatory compliance.
Product Specifications for AOD-9604 (99% Purity)
The following physicochemical and analytical specifications define our research‑grade AOD-9604. Each batch is verified against these parameters, and a comprehensive Certificate of Analysis (CoA) is issued for every lot.
- Sequence: Tyr‑Arg‑Ile‑Gln‑Leu‑Ala‑Gly‑Leu‑NH₂ (single‑letter code: Y‑R‑I‑Q‑L‑A‑G‑L‑NH₂), representing eight amino acids with a C‑terminal amide.
- Molecular weight: 1166.39 g/mol (monoisotopic mass).
- CAS number: 221231‑10‑3 (referenced in chemical literature and supplier databases).
- Appearance: White to off‑white lyophilized (freeze‑dried) powder.
- Solubility: Freely soluble in water, dilute acetic acid, or phosphate‑buffered saline; typically reconstituted at concentrations from 0.1 to 1 mg/mL for research protocols.
- Purity: ≥99% as measured by reverse‑phase high‑performance liquid chromatography (HPLC) at 214 nm.
- Packaging: Standard aliquots are 1 mg, 5 mg, 10 mg, and 100 mg in sterile glass vials. Larger bulk quantities (gram to kilogram scale) are provided in custom‑sized containers for high‑throughput projects or distributor networks.
- Storage recommendation: Upon receipt, store lyophilized peptide at –20 °C in a desiccated environment. After reconstitution, aliquot and freeze to avoid repeated freeze‑thaw cycles.
Analytical Data
To substantiate the 99% purity specification, each production lot is analyzed using a combination of chromatographic and spectrometric techniques. A representative HPLC chromatogram displays a single, symmetric peak at a retention time consistent with an in‑house AOD-9604 reference standard. The peak area integration typically exceeds 99%, with any minor peaks representing less than 0.5% individually. Mass spectrometry (ESI‑MS) confirms the intact molecular weight, and no significant peaks corresponding to synthesis‑related impurities or degradation products are observed. A Certificate of Analysis is generated for every batch and includes:
- HPLC purity and chromatogram
- Mass spectrum
- Peptide content (net peptide weight accounting for counter‑ions and residual moisture)
- Physical appearance
- Batch number and retest date
Customers may request a copy of the CoA prior to shipment or download it from a secure online portal. This transparency supports quality‑assured procurement for laboratories that require documented traceability.
Quality Control and Manufacturing
AOD-9604 is synthesized under controlled laboratory conditions using solid‑phase peptide synthesis (SPPS) with Fmoc chemistry. The process begins with a C‑terminal leucine amide pre‑loaded on resin, and the chain is elongated stepwise by coupling Fmoc‑protected amino acids. After cleavage and deprotection, the crude peptide is purified to ≥99% by preparative reverse‑phase HPLC using a gradient of acetonitrile in water containing 0.1% trifluoroacetic acid. The purified fraction is lyophilized to a dry powder and dispensed into sterile vials inside a class‑100 cleanroom environment.
Quality control is embedded at multiple stages:
- In‑process monitoring of coupling efficiency by ninhydrin testing or small‑scale cleavage analysis.
- Final QC by analytical HPLC, electrospray ionization mass spectrometry (ESI‑MS), and amino acid analysis to confirm composition and enantiomeric purity.
- Stability‑indicating tests under accelerated conditions (e.g., elevated temperature and humidity) to verify that the peptide maintains integrity during expected shipping and storage durations.
Only after a batch satisfactorily meets all release specifications is it approved for distribution. Retain samples are archived and periodically re‑analyzed to support extended stability claims.
Supply Chain and Sourcing
Our supply model relies on a direct partnership with a primary manufacturing facility, which eliminates unnecessary intermediaries and fosters transparent communication about raw materials, production schedules, and capacity planning. This relationship enables the following advantages for B2B customers:
- Security of supply: Long‑term manufacturing agreements and buffer stock of critical precursors reduce the risk of delays, even during periods of elevated demand.
- Raw material verification: All protected amino acid derivatives, resins, and solvents are procured from audited chemical suppliers who provide full documentation of origin, identity, and purity.
- Scalability: From milligram‑scale R&D quantities to kilogram‑scale commercial batches, the same validated purification method is used, ensuring consistent quality irrespective of order size.
- Cost efficiency: Bulk orders benefit from economies of scale, and we pass those savings on to academic core facilities, pharmaceutical screening groups, and regional distributors.
A rigorous vendor qualification program includes audits of the supplier’s quality system, review of batch records, and periodic site visits. This approach ensures that every gram of AOD-9604 shipped meets the same high standards as the initial sample evaluated by the customer.
Why Choose Our AOD-9604 (99%) for Your Research?
When selecting a peptide supplier for critical research programs, several factors beyond the listed purity become decisive. Our offer is built around the following pillars:
- Exceptional batch‑to‑batch consistency: Routine monitoring of process parameters and stringent release limits guarantee that HPLC purity ≥99% is maintained across years of production. Researchers can confidently compare data between experiments without questioning whether a shift in result stems from peptide variability.
- Flexible order scopes: Whether you need a single 1‑mg vial for a pilot study or hundreds of grams for a long‑running animal model investigation, we accommodate requests with tailored labeling, sterile packaging, and volume‑based pricing.
- Global logistics with temperature awareness: Lyophilized peptides are shipped at ambient temperature via express courier services that offer track‑and‑trace visibility. For large or time‑sensitive orders, we can arrange refrigerated packaging to mitigate exposure to extreme conditions. Most consignments reach major research hubs in North America, Europe, and Asia within 2–5 business days.
- Technical consultation: Our in‑house team includes scientists with peptide chemistry and cell biology expertise. They can advise on reconstitution protocols, solvent selection, and handling practices that preserve peptide stability in specific assay formats. While we do not provide guidance on biological interpretation or suggest applications, we help troubleshoot technical aspects of peptide use.
Frequently Asked Questions
What is the purity specification?
The purity is specified as ≥99% determined by reverse‑phase HPLC at 214 nm. The corresponding Certificate of Analysis details the precise value for each batch, typically exceeding 99% with minimal peak‑area contributions from impurities.
How is the product shipped?
AOD-9604 is supplied as a lyophilized powder in sterile, sealed glass vials. For short‑term transit, it is dispatched at ambient temperature, which does not compromise the dry peptide’s integrity. After reconstitution, it should be aliquoted and stored at –20 °C to –80 °C, depending on the experimental plan.
Can I request a Certificate of Analysis?
Yes. A CoA is included in the shipment container or made available electronically before dispatch. If an extra copy is needed for regulatory or auditing purposes, it can be provided upon request.
What if I require a modified sequence or a different quantity?
Custom peptide synthesis is available for research groups exploring structure‑activity relationships, such as substitution analogs or labeled variants of AOD-9604. Please contact our technical team with the desired sequence, modification, and purity specifications to discuss feasibility and lead times.
Is the peptide suitable for cell culture or animal studies?
Our AOD-9604 is prepared without preservatives and with low endotoxin levels (verified upon request). Researchers have used it in various cellular assays and preclinical models; however, specific suitability for a given protocol must be determined by the end‑user. Additional purification (e.g., sterile filtration after reconstitution) is recommended for aseptic work.
Research use only: All AOD-9604 products described here are intended exclusively for laboratory research as defined by applicable laws and regulations. They are not for use in humans, clinical applications, or veterinary medicine. No statements made in this document should be interpreted as a recommendation for therapeutic or diagnostic purposes.
For research use only. Not for human or veterinary use.
