Overview of DSIP Peptide
Delta Sleep-Inducing Peptide (DSIP) is a naturally occurring nonapeptide first isolated from the cerebral cortex of rabbits in 1977 by a research group at the University of Basel. The sequence consists of nine amino acids linked by peptide bonds, and it has been the subject of extensive neurobiological investigation ever since. In laboratory settings, DSIP is utilized exclusively to explore central nervous system regulatory mechanisms, with no intended application outside of controlled research environments.
The peptide is supplied as a lyophilized white to off-white powder, packaged under inert gas to preserve chemical integrity. Researchers procure DSIP bulk powder for research to study its interactions with neural receptors, its role in sleep architecture modulation, and its influence on endocrine stress pathways. All experimental work is conducted in vitro or using authorized animal models, adhering to institutional and governmental guidelines for the ethical use of research compounds.
- Isolated from rabbit cerebral cortex; later detected in human plasma, cerebrospinal fluid, and various brain regions.
- Predominantly investigated in neurobiology, endocrinology, and circadian rhythm studies.
- Provided as a sterile, lyophilized powder strictly for laboratory research use only.
Chemical and Physical Properties
DSIP carries the molecular formula C35H48N10O15 and a calculated molecular weight of 848.8 g/mol. The primary structure is H-Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu-OH, indicating a free carboxyl terminus at the glutamic acid residue. This peptide is freely soluble in water and commonly used aqueous buffers such as phosphate‑buffered saline (PBS) pH 7.4. For reconstitution, sterile PBS or sterile water for injection (WFI) is recommended to prepare stock solutions at concentrations appropriate for the experimental design.
Physically, the lyophilized material is a light, fluffy powder with no distinct odor. The CAS registry number 62568‑57‑4 is often used for cataloging and import/export documentation. The peptide is stable when stored dry and protected from moisture and light; however, in solution it is susceptible to enzymatic degradation and aggregation, so researchers must handle it with appropriate aseptic technique and use it promptly.
- Molecular formula: C35H48N10O15
- Molecular weight: 848.8 g/mol
- Sequence: H-Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu-OH
- Solubility: Soluble in water and aqueous buffers; reconstitution in sterile PBS is standard.
- CAS number: 62568‑57‑4
Research Applications of DSIP
DSIP is extensively employed in experimental models to dissect the neurochemical substrates of sleep–wake regulation. Electroencephalographic (EEG) recordings in rodents administered DSIP reveal shifts in slow‑wave sleep patterns, making the peptide a valuable tool for studying sleep homeostasis, circadian rhythmicity, and the interplay between sleep and metabolic processes. These investigations help to clarify fundamental neural circuits without implying any translational outcome.
Beyond sleep research, DSIP serves as a probe in stress physiology. Laboratory studies have examined its modulatory effect on the hypothalamic‑pituitary‑adrenal (HPA) axis, including changes in adrenocorticotropic hormone (ACTH) and corticosterone levels under stress paradigms. In vitro binding assays and cell‑based models further enable investigation of signal transduction pathways—such as cyclic AMP (cAMP) modulation and calcium flux—potentially linked to DSIP‑receptor interactions. Peptide‑receptor cross‑talk with other neurotransmitter systems (e.g., GABAergic, opioidergic) is also an active field of inquiry.
Other areas of approved laboratory usage include:
- Assessment of neuroendocrine synchronization in seasonal breeding animal models.
- Study of antioxidant and anti‑apoptotic cellular responses under oxidative stress conditions in neuronal cultures.
- Mapping of peptide distribution in brain tissue using immunohistochemistry and mass spectrometry imaging.
Quality and Purity Specifications
When sourcing DSIP bulk powder for research, rigorous analytical criteria ensure reproducibility. Each lot undergoes high‑performance liquid chromatography (HPLC) to confirm purity ≥98%, with the main peak area meeting this threshold relative to all integrated peaks. The identity of the peptide is verified by electrospray ionization mass spectrometry (ESI‑MS) or matrix‑assisted laser desorption/ionization (MALDI‑MS), and the sequence is confirmed through tandem MS fragmentation.
Net peptide content is determined by quantitative amino acid analysis, accounting for counter‑ions and residual moisture. Karl Fischer titration or loss‑on‑drying methods report water content, typically ≤10%, to avoid overestimation of active material. Endotoxin levels, critical for cell‑based assays and in‑vivo models, are measured using the Limulus amebocyte lysate (LAL) assay, with acceptance criterion <1 EU/μg. Sterility is evaluated according to compendial methods (e.g., USP membrane filtration). Additional tests often include residual trifluoroacetic acid (TFA) quantification and heavy metal analysis, with results documented in the batch‑specific Certificate of Analysis (CoA).
- Purity (HPLC): ≥98%
- Peptide content: Reported as net peptide weight, corrected for water and counter‑ions.
- Endotoxin: <1 EU/μg by LAL method.
- Sterility: Tested negative following membrane filtration.
- Appearance: White to off‑white lyophilized powder.
Handling and Storage Guidelines
Proper storage of DSIP is paramount for maintaining peptide stability and experimental consistency. The lyophilized powder should be kept in a tightly sealed container at –20 °C, desiccated, and protected from direct light. When kept under these conditions, the peptide typically retains its specified purity for up to two years from the date of manufacture. Upon receipt, researchers are advised to briefly centrifuge the vial before opening to collect any powder displaced during transit.
For reconstitution, sterile PBS or sterile water is added to achieve the desired stock concentration (commonly 1 mg/mL). Gentle swirling is preferred over vortexing to avoid shear‑induced aggregation. Once in solution, DSIP should be immediately aliquoted into single‑use portions in low‑protein‑binding microcentrifuge tubes and stored at –80 °C. Repeated freeze‑thaw cycles lead to peptide degradation and loss of bioactivity; therefore, each aliquot should be thawed only once immediately prior to use. All handling steps must be performed using aseptic technique in a biosafety cabinet to preserve sterility and endotoxin‑low status.
- Store lyophilized powder at –20 °C, desiccated, protected from light.
- Reconstitute with sterile PBS or sterile water; avoid vigorous agitation.
- Aliquot reconstituted peptide and store at –80 °C.
- Use one aliquot per experiment; discard any unused portion.
- Do not refreeze thawed aliquots.
Ordering DSIP Bulk Powder
DSIP bulk powder for research is supplied in sealed glass vials with a crimp‑cap closure, often under argon or nitrogen headspace to prevent oxidation. Standard bulk quantities include 10 mg, 50 mg, and 100 mg per vial; larger custom packaging may be arranged upon inquiry to accommodate high‑throughput screening or long‑term study protocols. Each shipment is accompanied by a detailed Certificate of Analysis that includes HPLC chromatograms, mass spectrometry data, net peptide content, endotoxin results, and sterility test summaries, enabling researchers to verify batch‑to‑batch consistency.
Lyophilized DSIP is generally stable at ambient temperature for short‑term shipping, so vials are dispatched without cold packs unless the destination country imposes veterinary or inspection requirements. For reconstituted liquid samples—rarely shipped due to stability concerns—dry ice packaging is employed to maintain –80 °C conditions throughout transit. International orders are packed to comply with customs regulations, clearly labeled as non‑hazardous research chemicals, and accompanied by a declaration stating the material is for laboratory research use only. Buyers should confirm their institution’s import permits and applicable national regulations before placing an order.
- Standard formats: 10 mg, 50 mg, 100 mg lyophilized in sealed glass vials.
- Custom bulk quantities available on request.
- Batch‑specific Certificate of Analysis with HPLC, MS, and purity data included.
- Ambient shipping for lyophilized powder; dry ice for reconstituted solutions if necessary.
- International delivery with research‑use‑only customs documentation.
Avis « À usage exclusif de la recherche » : DSIP bulk powder, and all associated analytical services, are intended strictly for laboratory research purposes. This product is not manufactured or sold for any diagnostic, therapeutic, or prophylactic application in humans or animals. Researchers must adhere to all applicable laws, regulations, and institutional guidelines governing the use of research peptides. Any use outside of controlled laboratory investigations is explicitly not supported by the manufacturer or distributor.
Réservé à la recherche. Ne pas utiliser chez l'homme ni chez les animaux.
